RRC ID 49587
著者 Ríos-Barrera LD, Sigurbjörnsdóttir S, Baer M, Leptin M.
タイトル Dual function for Tango1 in secretion of bulky cargo and in ER-Golgi morphology.
ジャーナル Proc Natl Acad Sci U S A
Abstract Tango1 enables ER-to-Golgi trafficking of large proteins. We show here that loss of Tango1, in addition to disrupting protein secretion and ER/Golgi morphology, causes ER stress and defects in cell shape. We find that the previously observed dependence of smaller cargos on Tango1 is a secondary effect. If large cargos like Dumpy, which we identify as a Tango1 cargo, are removed from the cell, nonbulky proteins reenter the secretory pathway. Removal of blocking cargo also restores cell morphology and attenuates the ER-stress response. Thus, failures in the secretion of nonbulky proteins, ER stress, and defective cell morphology are secondary consequences of bulky cargo retention. By contrast, ER/Golgi defects in Tango1-depleted cells persist in the absence of bulky cargo, showing that they are due to a secretion-independent function of Tango1. Therefore, maintenance of ER/Golgi architecture and bulky cargo transport are the primary functions for Tango1.
巻・号 114(48)
ページ E10389-E10398
公開日 2017-11-28
DOI 10.1073/pnas.1711408114
PII 1711408114
PMID 29138315
PMC PMC5715762
MeSH Animals Aryl Hydrocarbon Receptor Nuclear Translocator / physiology* Drosophila Proteins / physiology* Drosophila melanogaster Endoplasmic Reticulum / physiology* Endoplasmic Reticulum Stress / physiology Gene Knockdown Techniques Golgi Apparatus / physiology* Mutagenesis Protein Transport / physiology Vesicular Transport Proteins / physiology*
IF 9.412
引用数 15
リソース情報
ショウジョウバエ 11098R-3 16858R-1