RRC ID 35587
著者 Ollivierre JN, Sikora JL, Beuning PJ.
タイトル Dimer exchange and cleavage specificity of the DNA damage response protein UmuD.
ジャーナル Biochim Biophys Acta
Abstract The cellular response to DNA damage in Escherichia coli is controlled in part by the activity of the umuD gene products. The full-length dimeric UmuD(2) is the initial product that is expressed shortly after the induction of the SOS response and inhibits bacterial mutagenesis, allowing for error-free repair to occur. Over time, the slow auto-cleavage of UmuD(2) to UmuD'(2) promotes mutagenesis to ensure cell survival. The intracellular levels of UmuD(2) and UmuD'(2) are further regulated by degradation in vivo, returning the cell to a non-mutagenic state. To further understand the dynamic regulatory roles of the umuD gene products, we monitored the kinetics of exchange and cleavage of the UmuD(2) and UmuD'(2) homodimers as well as of the UmuDD' heterodimer under equilibrium conditions. We found that the heterodimer is the preferred but not exclusive protein form, and that both the heterodimer and homodimers exhibit slow exchange kinetics which is further inhibited in the presence of interacting partner DinB. In addition, the heterodimer efficiently cleaves to form UmuD'(2). Together, this work reveals an intricate UmuD lifecycle that involves dimer exchange and cleavage in the regulation of the DNA damage response.
巻・号 1834(2)
ページ 611-20
公開日 2013-2-1
DOI 10.1016/j.bbapap.2012.11.008
PII S1570-9639(12)00265-8
PMID 23220418
MeSH DNA Damage* DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Bacterial / metabolism* DNA-Directed DNA Polymerase / chemistry DNA-Directed DNA Polymerase / genetics DNA-Directed DNA Polymerase / metabolism* Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Kinetics Mutagenesis Protein Multimerization / physiology*
IF 3.411
引用数 9
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) NA