RRC ID 47919
著者 Frenkel L, Muraro NI, Beltrán González AN, Marcora MS, Bernabó G, Hermann-Luibl C, Romero JI, Helfrich-Förster C, Castaño EM, Marino-Busjle C, Calvo DJ, Ceriani MF.
タイトル Organization of Circadian Behavior Relies on Glycinergic Transmission.
ジャーナル Cell Rep
Abstract The small ventral lateral neurons (sLNvs) constitute a central circadian pacemaker in the Drosophila brain. They organize daily locomotor activity, partly through the release of the neuropeptide pigment-dispersing factor (PDF), coordinating the action of the remaining clusters required for network synchronization. Despite extensive efforts, the basic principles underlying communication among circadian clusters remain obscure. We identified classical neurotransmitters released by sLNvs through disruption of specific transporters. Adult-specific RNAi-mediated downregulation of the glycine transporter or impairment of glycine synthesis in LNv neurons increased period length by nearly an hour without affecting rhythmicity of locomotor activity. Electrophysiological recordings showed that glycine reduces spiking frequency in circadian neurons. Interestingly, downregulation of glycine receptor subunits in specific sLNv targets impaired rhythmicity, revealing involvement of glycine in information processing within the network. These data identify glycinergic inhibition of specific targets as a cue that contributes to the synchronization of the circadian network.
巻・号 19(1)
ページ 72-85
公開日 2017-4-4
DOI 10.1016/j.celrep.2017.03.034
PII S2211-1247(17)30366-2
PMID 28380364
MeSH Animals Animals, Genetically Modified Brain / metabolism Circadian Rhythm / physiology* Down-Regulation Drosophila Proteins / metabolism Drosophila melanogaster / metabolism Glycine / metabolism* Glycine Plasma Membrane Transport Proteins / genetics Glycine Plasma Membrane Transport Proteins / metabolism* Humans Neurons / metabolism Neuropeptides / metabolism Neurotransmitter Agents / metabolism RNA Interference Receptors, Glycine / genetics Receptors, Glycine / metabolism* Synaptic Transmission*
IF 8.109
引用数 25
WOS 分野 CELL BIOLOGY
リソース情報
ショウジョウバエ 1732R-1 1732R-3 3159R-2 5526R-3 15444R-1 15444R-2