RRC ID 35593
著者 Ryo M, Oshikoshi Y, Doi S, Motoki S, Niimi A, Aoki S.
タイトル Firefly luciferase as the reporter for transcriptional response to the environment in Escherichia coli.
ジャーナル Anal Biochem
Abstract We demonstrate that firefly luciferase is a good reporter in Escherichia coli for transcription dynamics in response to the environment. E. coli strains, carrying a fusion of the promoter of the ycgZ gene and the coding region of the luciferase gene, showed transient bioluminescence on receiving blue light. This response was compromised in mutants lacking known regulators in manners consistent with each regulator's function. We also show that relA, a gene encoding a (p)ppGpp synthetase, affects ycgZ dynamics when nullified. Moreover, two unstable luciferase variants showed improved response dynamics and should be useful to study quick changes of gene expression.
巻・号 443(2)
ページ 211-3
公開日 2013-12-15
DOI 10.1016/j.ab.2013.08.025
PII S0003-2697(13)00409-0
PMID 24012794
MeSH Animals Escherichia coli / genetics* Fireflies / enzymology* Gene Expression Regulation, Bacterial Genes, Bacterial / genetics* Genes, Reporter* Light Luciferases, Firefly / genetics* Transcriptional Activation*
IF 2.877
引用数 1
WOS 分野 CHEMISTRY, ANALYTICAL BIOCHEMISTRY & MOLECULAR BIOLOGY BIOCHEMICAL RESEARCH METHODS
リソース情報
原核生物(大腸菌) ME9062(BW25113) JW1149-KC JW1150-KC JW5437-KC JW2755-KC