RRC ID 48547
著者 Kawazura T, Matsumoto K, Kojima K, Kato F, Kanai T, Niki H, Shiomi D.
タイトル Exclusion of assembled MreB by anionic phospholipids at cell poles confers cell polarity for bidirectional growth.
ジャーナル Mol Microbiol
Abstract Cell polarity determines the direction of cell growth in bacteria. MreB actin spatially regulates peptidoglycan synthesis to enable cells to elongate bidirectionally. MreB densely localizes in the cylindrical part of the rod cell and not in polar regions in Escherichia coli. When treated with A22, which inhibits MreB polymerization, rod-shaped cells became round and MreB was diffusely distributed throughout the cytoplasmic membrane. A22 removal resulted in restoration of the rod shape. Initially, diffuse MreB started to re-assemble, and MreB-free zones were subsequently observed in the cytoplasmic membrane. These MreB-free zones finally became cell poles, allowing the cells to elongate bidirectionally. When MreB was artificially located at the cell poles, an additional pole was created, indicating that artificial localization of MreB at the cell pole induced local peptidoglycan synthesis. It was found that the anionic phospholipids (aPLs), phosphatidylglycerol and cardiolipin, which were enriched in cell poles preferentially interact with monomeric MreB compared with assembled MreB in vitro. MreB tended to localize to cell poles in cells lacking both aPLs, resulting in production of Y-shaped cells. Their findings indicated that aPLs exclude assembled MreB from cell poles to establish cell polarity, thereby allowing cells to elongate in a particular direction.
巻・号 104(3)
ページ 472-486
公開日 2017-5-1
DOI 10.1111/mmi.13639
PMID 28164388
MeSH Cell Membrane / metabolism Cell Polarity / physiology* Cell Shape / physiology Cell Wall / metabolism Escherichia coli / cytology* Escherichia coli / drug effects Escherichia coli / growth & development Escherichia coli / metabolism Escherichia coli Proteins / metabolism* Phospholipids / metabolism* Polymerization Thiourea / analogs & derivatives Thiourea / pharmacology
IF 3.418
引用数 19
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9796 ME9797 ME9798 ME9799 ME9800 ME9801 ME9802 ME9803 ME9804 ME9805