RRC ID 34134
著者 Hayashi H, Kurusu Y.
タイトル DNA regions responsible for maintenance of Shingobium plasmid pYAN-2.
ジャーナル Microbes Environ
Abstract We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli.
巻・号 29(1)
ページ 96-9
公開日 2014-1-1
DOI 10.1264/jsme2.me13135
PII DN/JST.JSTAGE/jsme2/ME13135
PMID 24441514
PMC PMC4041236
MeSH Alphaproteobacteria / genetics* Bacterial Proteins / genetics Base Sequence DNA, Bacterial / genetics* Escherichia coli / genetics Gene Dosage Molecular Sequence Data Plasmids / genetics*
IF 2.575
引用数 1
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 7371 JCM 7452 JCM 11777