RRC ID 47427
著者 Chen J, Reiher W, Hermann-Luibl C, Sellami A, Cognigni P, Kondo S, Helfrich-Förster C, Veenstra JA, Wegener C.
タイトル Allatostatin A Signalling in Drosophila Regulates Feeding and Sleep and Is Modulated by PDF.
ジャーナル PLoS Genet
Abstract Feeding and sleep are fundamental behaviours with significant interconnections and cross-modulations. The circadian system and peptidergic signals are important components of this modulation, but still little is known about the mechanisms and networks by which they interact to regulate feeding and sleep. We show that specific thermogenetic activation of peptidergic Allatostatin A (AstA)-expressing PLP neurons and enteroendocrine cells reduces feeding and promotes sleep in the fruit fly Drosophila. The effects of AstA cell activation are mediated by AstA peptides with receptors homolog to galanin receptors subserving similar and apparently conserved functions in vertebrates. We further identify the PLP neurons as a downstream target of the neuropeptide pigment-dispersing factor (PDF), an output factor of the circadian clock. PLP neurons are contacted by PDF-expressing clock neurons, and express a functional PDF receptor demonstrated by cAMP imaging. Silencing of AstA signalling and continuous input to AstA cells by tethered PDF changes the sleep/activity ratio in opposite directions but does not affect rhythmicity. Taken together, our results suggest that pleiotropic AstA signalling by a distinct neuronal and enteroendocrine AstA cell subset adapts the fly to a digestive energy-saving state which can be modulated by PDF.
巻・号 12(9)
ページ e1006346
公開日 2016-9-1
DOI 10.1371/journal.pgen.1006346
PII PGENETICS-D-16-00644
PMID 27689358
PMC PMC5045179
IF 5.175
引用数 41
WOS 分野 GENETICS & HEREDITY
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