RRC ID 34061
著者 Shintani M, Matsui K, Inoue J, Hosoyama A, Ohji S, Yamazoe A, Nojiri H, Kimbara K, Ohkuma M.
タイトル Single-cell analyses revealed transfer ranges of IncP-1, IncP-7, and IncP-9 plasmids in a soil bacterial community.
ジャーナル Appl Environ Microbiol
Abstract The conjugative transfer ranges of three different plasmids of the incompatibility groups IncP-1 (pBP136), IncP-7 (pCAR1), and IncP-9 (NAH7) were investigated in soil bacterial communities by culture-dependent and culture-independent methods. Pseudomonas putida, a donor of each plasmid, was mated with soil bacteria, and green fluorescent protein (GFP), encoded on the plasmid, was used as a reporter protein for successful transfer. GFP-expressing transconjugants were detected and separated at the single-cell level by flow cytometry. Each cell was then analyzed by PCR and sequencing of its 16S rRNA gene following either whole-genome amplification or cultivation. A large number of bacteria within the phylum Proteobacteria was identified as transconjugants for pBP136 by both culture-dependent and culture-independent methods. Transconjugants belonging to the phyla Actinobacteria, Bacteroidetes, and Firmicutes were detected only by the culture-independent method. Members of the genus Pseudomonas (class Gammaproteobacteria) were identified as major transconjugants of pCAR1 and NAH7 by both methods, whereas Delftia species (class Betaproteobacteria) were detected only by the culture-independent method. The transconjugants represented a minority of the soil bacteria. Although pCAR1-containing Delftia strains could not be cultivated after a one-to-one filter mating assay between the donor and cultivable Delftia strains as recipients, fluorescence in situ hybridization detected pCAR1-containing Delftia cells, suggesting that Delftia was a "transient" host of pCAR1.
巻・号 80(1)
ページ 138-45
公開日 2014-1-1
DOI 10.1128/AEM.02571-13
PII AEM.02571-13
PMID 24141122
PMC PMC3911017
MeSH Bacteria / classification* Bacteria / genetics* Conjugation, Genetic* DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Flow Cytometry Gene Transfer, Horizontal* Genes, Reporter Green Fluorescent Proteins / analysis Molecular Sequence Data Plasmids* Polymerase Chain Reaction RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Single-Cell Analysis Soil Microbiology*
IF 4.016
引用数 40
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 1090 JCM 1236 JCM 1237 JCM 1677 JCM 5833 JCM 6203 JCM 13063 JCM 13333 JCM 20371 JCM 20682