RRC ID |
40738
|
著者 |
Bottacini F, O'Connell Motherway M, Casey E, McDonnell B, Mahony J, Ventura M, van Sinderen D.
|
タイトル |
Discovery of a conjugative megaplasmid in Bifidobacterium breve.
|
ジャーナル |
Appl Environ Microbiol
|
Abstract |
Bifidobacterium breve is a common and sometimes very abundant inhabitant of the human gut. Genome sequencing of B. breve JCM 7017 revealed the presence of an extrachromosomal element, designated pMP7017 consisting of >190 kb, thus representing the first reported bifidobacterial megaplasmid. In silico characterization of this element revealed several genomic features supporting a stable establishment of the megaplasmid in its host, illustrated by predicted CRISPR-Cas functions that are known to protect the host against intrusion of foreign DNA. Interestingly, pMP7017 is also predicted to encode a conjugative DNA transfer apparatus and, consistent with this notion, we demonstrate here the conjugal transfer of pMP7017 to representative strains of B. breve and B. longum subsp. longum. We also demonstrate the presence of a megaplasmid with homology to pMP7017 in three B. longum subsp. longum strains.
|
巻・号 |
81(1)
|
ページ |
166-76
|
公開日 |
2015-1-1
|
DOI |
10.1128/AEM.02871-14
|
PII |
AEM.02871-14
|
PMID |
25326305
|
PMC |
PMC4272746
|
MeSH |
Bifidobacterium / genetics*
CRISPR-Cas Systems
Conjugation, Genetic
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Genes, Bacterial
Molecular Sequence Data
Plasmids*
Sequence Analysis, DNA
|
IF |
4.016
|
引用数 |
13
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 7017
JCM 7019 |