RRC ID |
33714
|
著者 |
Ren W, Zhang Y, Li M, Wu L, Wang G, Baeg GH, You J, Li Z, Lin X.
|
タイトル |
Windpipe controls Drosophila intestinal homeostasis by regulating JAK/STAT pathway via promoting receptor endocytosis and lysosomal degradation.
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ジャーナル |
PLoS Genet
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Abstract |
The adult intestinal homeostasis is tightly controlled by proper proliferation and differentiation of intestinal stem cells. The JAK/STAT (Janus Kinase/Signal Transducer and Activator of Transcription) signaling pathway is essential for the regulation of adult stem cell activities and maintenance of intestinal homeostasis. Currently, it remains largely unknown how JAK/STAT signaling activities are regulated in these processes. Here we have identified windpipe (wdp) as a novel component of the JAK/STAT pathway. We demonstrate that Wdp is positively regulated by JAK/STAT signaling in Drosophila adult intestines. Loss of wdp activity results in the disruption of midgut homeostasis under normal and regenerative conditions. Conversely, ectopic expression of Wdp inhibits JAK/STAT signaling activity. Importantly, we show that Wdp interacts with the receptor Domeless (Dome), and promotes its internalization for subsequent lysosomal degradation. Together, these data led us to propose that Wdp acts as a novel negative feedback regulator of the JAK/STAT pathway in regulating intestinal homeostasis.
|
巻・号 |
11(4)
|
ページ |
e1005180
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公開日 |
2015-4-1
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DOI |
10.1371/journal.pgen.1005180
|
PII |
PGENETICS-D-15-00063
|
PMID |
25923769
|
PMC |
PMC4414558
|
MeSH |
Animals
Cell Differentiation / genetics*
Cell Proliferation
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism
Drosophila melanogaster
Endocytosis / genetics
Gene Expression Regulation, Developmental
Homeostasis / genetics
Intestinal Mucosa / metabolism*
Intestines / growth & development
Janus Kinases / genetics*
Janus Kinases / metabolism
Lysosomes / genetics
Lysosomes / metabolism
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Receptors, Interleukin / genetics*
Receptors, Interleukin / metabolism
STAT Transcription Factors / genetics
STAT Transcription Factors / metabolism
Signal Transduction
Stem Cells
|
IF |
5.175
|
引用数 |
75
|
WOS 分野
|
GENETICS & HEREDITY
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リソース情報 |
ショウジョウバエ |
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