RRC ID 33548
著者 Fukushima T, Tanabe T, Yamamoto H, Hosoya S, Sato T, Yoshikawa H, Sekiguchi J.
タイトル Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
ジャーナル J Biochem
Abstract The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is transcribed by EsigmaE RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the forespores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation.
巻・号 136(3)
ページ 283-91
公開日 2004-9-1
DOI 10.1093/jb/mvh151
PII 136/3/283
PMID 15598884
MeSH Amidohydrolases / genetics* Amidohydrolases / metabolism Bacillus subtilis / genetics* Bacillus subtilis / metabolism* Bacterial Proteins / chemistry Base Sequence Blotting, Northern Cell Survival Centrifugation, Density Gradient DNA Primers / chemistry DNA-Binding Proteins / chemistry DNA-Directed RNA Polymerases / chemistry Escherichia coli / metabolism Genetic Complementation Test Genotype Green Fluorescent Proteins / metabolism Microscopy, Electron Microscopy, Phase-Contrast Molecular Sequence Data Mutation Picolinic Acids / chemistry Plasmids / metabolism Polysaccharides / chemistry RNA, Messenger / metabolism Sequence Homology, Amino Acid Spores, Bacterial / metabolism* Time Factors Transcription Factors / chemistry
IF 2.476
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS56 MBS57 MBS58