論文 - 詳細
| RRC ID | 33551 |
|---|---|
| 著者 | Satomura T, Shimura D, Asai K, Sadaie Y, Hirooka K, Fujita Y. |
| タイトル | Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system. |
| ジャーナル | J Bacteriol |
| Abstract |
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively. |
| 巻・号 | 187(14) |
| ページ | 4813-21 |
| 公開日 | 2005-7-1 |
| DOI | 10.1128/JB.187.14.4813-4821.2005 |
| PII | 187/14/4813 |
| PMID | 15995196 |
| PMC | PMC1169493 |
| MeSH | Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Base Sequence Carrier Proteins / genetics Carrier Proteins / metabolism* DNA Primers Genes, Reporter Genotype Glutaminase / genetics Glutaminase / metabolism* Glutamine / metabolism* Histidine Kinase Kinetics Molecular Sequence Data Protein Kinases / genetics Protein Kinases / metabolism* Trans-Activators / genetics Trans-Activators / metabolism* beta-Galactosidase / genetics beta-Galactosidase / metabolism |
| IF | 3.006 |
| 引用数 | 30 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(枯草菌) | MBS270 MBS271 MBS294 |