RRC ID 33562
著者 Inaoka T, Satomura T, Fujita Y, Ochi K.
タイトル Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
ジャーナル FEMS Microbiol Lett
Abstract Bacillus subtilis GlcP regulates a secondary metabolism, the neotrehalosadiamine synthesis pathway, by repressing a neotrehalosadiamine biosynthesis operon in response to glucose present in the medium. Here, we investigated, by use of transcriptome, additional effects of glcP disruption on other gene expression. In the GlcP-null mutant, the expression of alsSD and maeN was decreased, while the expression of licBCAH, ntdABC, yyaH-maa, and yyaJ was increased. The effect caused by loss of GlcP function was, however, completely negated in a mutant lacking the ability to synthesize neotrehalosadiamine. Moreover, addition of neotrehalosadiamine into the growth medium had no effect on the expression of these genes, indicating that GlcP-promoted regulation was exerted depending on de novo neotrehalosadiamine synthesis rather than neotrehalosadiamine per se. These findings suggest that GlcP participates in regulation of certain genes by repressing the neotrehalosadiamine biosynthesis operon. This novel regulation system may provide new insights into study of B. subtilis gene expression.
巻・号 291(2)
ページ 151-6
公開日 2009-2-1
DOI 10.1111/j.1574-6968.2008.01450.x
PII FML1450
PMID 19087206
MeSH Anti-Bacterial Agents / metabolism Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Gene Expression Regulation, Bacterial* Glucose / metabolism Operon Promoter Regions, Genetic Trehalose / analogs & derivatives Trehalose / biosynthesis
IF 1.987
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS155 MBS297