RRC ID 17485
著者 Yano H, Deckert GE, Rogers LM, Top EM.
タイトル Roles of long and short replication initiation proteins in the fate of IncP-1 plasmids.
ジャーナル J Bacteriol
Abstract Broad-host-range IncP-1 plasmids generally encode two replication initiation proteins, TrfA1 and TrfA2. TrfA2 is produced from an internal translational start site within trfA1. While TrfA1 was previously shown to be essential for replication in Pseudomonas aeruginosa, its role in other bacteria within its broad host range has not been established. To address the role of TrfA1 and TrfA2 in other hosts, efficiency of transformation, plasmid copy number (PCN), and plasmid stability were first compared between a mini-IncP-1β plasmid and its trfA1 frameshift variant in four phylogenetically distant hosts: Escherichia coli, Pseudomonas putida, Sphingobium japonicum, and Cupriavidus necator. TrfA2 was sufficient for replication in these hosts, but the presence of TrfA1 enhanced transformation efficiency and PCN. However, TrfA1 did not contribute to, and even negatively affected, long-term plasmid persistence. When trfA genes were cloned under a constitutive promoter in the chromosomes of the four hosts, strains expressing either both TrfA1 and TrfA2 or TrfA1 alone, again, generally elicited a higher PCN of an IncP1-β replicon than strains expressing TrfA2 alone. When a single species of TrfA was produced at different concentrations in E. coli cells, TrfA1 maintained a 3- to 4-fold higher PCN than TrfA2 at the same TrfA concentrations, indicating that replication mediated by TrfA1 is more efficient than that by TrfA2. These results suggest that the broad-host-range properties of IncP-1 plasmids are essentially conferred by TrfA2 and the intact replication origin alone but that TrfA1 is nonetheless important to efficiently establish plasmid replication upon transfer into a broad range of hosts.
巻・号 194(6)
ページ 1533-43
公開日 2012-3-1
DOI 10.1128/JB.06395-11
PII JB.06395-11
PMID 22228734
PMC PMC3294859
MeSH DNA Replication* DNA, Bacterial / genetics DNA, Bacterial / metabolism* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Genomic Instability Gram-Negative Bacteria / genetics* Gram-Negative Bacteria / metabolism* Plasmids* Transformation, Bacterial
IF 3.006
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113)