RRC ID 28134
著者 Xu L, Carrie C, Law SR, Murcha MW, Whelan J.
タイトル Acquisition, conservation, and loss of dual-targeted proteins in land plants.
ジャーナル Plant Physiol
Abstract The dual-targeting ability of a variety of proteins from Physcomitrella patens, rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana) was tested to determine when dual targeting arose and to what extent it was conserved in land plants. Overall, the targeting ability of over 80 different proteins from rice and P. patens, representing 42 dual-targeted proteins in Arabidopsis, was tested. We found that dual targeting arose early in land plant evolution, as it was evident in many cases with P. patens proteins that were conserved in rice and Arabidopsis. Furthermore, we found that the acquisition of dual-targeting ability is still occurring, evident in P. patens as well as rice and Arabidopsis. The loss of dual-targeting ability appears to be rare, but does occur. Ascorbate peroxidase represents such an example. After gene duplication in rice, individual genes encode proteins that are targeted to a single organelle. Although we found that dual targeting was generally conserved, the ability to detect dual-targeted proteins differed depending on the cell types used. Furthermore, it appears that small changes in the targeting signal can result in a loss (or gain) of dual-targeting ability. Overall, examination of the targeting signals within this study did not reveal any clear patterns that would predict dual-targeting ability. The acquisition of dual-targeting ability also appears to be coordinated between proteins. Mitochondrial intermembrane space import and assembly protein40, a protein involved in oxidative folding in mitochondria and peroxisomes, provides an example where acquisition of dual targeting is accompanied by the dual targeting of substrate proteins.
巻・号 161(2)
ページ 644-62
公開日 2013-2-1
DOI 10.1104/pp.112.210997
PII pp.112.210997
PMID 23257241
PMC PMC3561010
MeSH Arabidopsis / genetics Arabidopsis / metabolism* Ascorbate Peroxidases / classification Ascorbate Peroxidases / genetics Ascorbate Peroxidases / metabolism Bryopsida / genetics Bryopsida / metabolism* Evolution, Molecular Hexokinase / classification Hexokinase / genetics Hexokinase / metabolism Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Mitochondria / metabolism NADH, NADPH Oxidoreductases / classification NADH, NADPH Oxidoreductases / genetics NADH, NADPH Oxidoreductases / metabolism Organelles / metabolism* Oryza / genetics Oryza / metabolism* Peroxisomes / metabolism Phylogeny Plant Proteins / classification Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Plastids / metabolism Protein Transport / genetics
IF 6.902
引用数 49
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst00020