RRC ID 30993
著者 Ohyama Y, Ito H, Kobayashi Y, Ikka T, Morita A, Kobayashi M, Imaizumi R, Aoki T, Komatsu K, Sakata Y, Iuchi S, Koyama H.
タイトル Characterization of AtSTOP1 orthologous genes in tobacco and other plant species.
ジャーナル Plant Physiol
Abstract Aluminum (Al) and proton (H⁺) tolerances are essential traits for plants to adapt to acid soil environments. In Arabidopsis (Arabidopsis thaliana), these tolerances are mediated by a zinc-finger transcription factor, SENSITIVE TO PROTON RHIZOTOXICITY1 (AtSTOP1), which regulates the transcription of multiple genes critical for tolerance to both stressors. Here, the functions of orthologous proteins (STOP1-like proteins) in other plant species were characterized by reverse genetics analyses and in planta complementation assays. RNA interference of a gene for NtSTOP1 repressed Al and H⁺ tolerances of tobacco (Nicotiana tabacum) roots. Tobacco roots released citrate in response to Al, concomitant with the up-regulated transcription of an ortholog of an Al tolerance gene encoding a citrate-transporting multidrug and toxic compound extrusion protein. The RNA interference repression of NtSTOP1 blocked this process and also repressed the transcription of another orthologous gene for Al tolerance, ALUMINUM SENSITIVE3, which encodes a prokaryote-type transporter. These results demonstrated that NtSTOP1 regulates Al tolerance in tobacco through the transcriptional regulation of these genes. The in planta complementation assays revealed that other plant species, including woody plants, a legume, and a moss (Physcomitrella patens), possess functional STOP1-like proteins that can activate several H⁺ and Al-tolerance genes in Arabidopsis. Knocking out the gene encoding the STOP1-like protein decreased the Al tolerance of P. patens. Together, our results strongly suggest that transcriptional regulation by STOP1-like proteins is evolutionarily conserved among land plants and that it confers the ability to survive in acid soils through the transcriptional regulation of Al- and H⁺-tolerance genes.
巻・号 162(4)
ページ 1937-46
公開日 2013-8-1
DOI 10.1104/pp.113.218958
PII pp.113.218958
PMID 23749850
PMC PMC3729772
MeSH Aluminum / toxicity Amino Acid Sequence Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Bryopsida / genetics Bryopsida / growth & development Citrates / metabolism Evolution, Molecular Gene Expression Regulation, Plant Gene Knockout Techniques Genetic Complementation Test Hydroponics Malates / metabolism Molecular Sequence Data Plant Proteins / genetics* Plant Proteins / isolation & purification Plant Proteins / metabolism Plant Roots / drug effects Plant Roots / genetics Plant Roots / metabolism Plants, Genetically Modified RNA Interference Soil Tobacco / drug effects Tobacco / genetics* Tobacco / physiology Transcription Factors / genetics* Transcription Factors / metabolism Zinc Fingers / genetics
IF 6.902
引用数 43
WOS 分野 PLANT SCIENCES
リソース情報
ミヤコグサ・ダイズ Lotus japonicus Sequence information