RRC ID 11413
著者 Anterola A, Göbel C, Hornung E, Sellhorn G, Feussner I, Grimes H.
タイトル Physcomitrella patens has lipoxygenases for both eicosanoid and octadecanoid pathways.
ジャーナル Phytochemistry
Abstract Mosses have substantial amounts of long chain C20 polyunsaturated fatty acids, such as arachidonic and eicosapentaenoic acid, in addition to the shorter chain C18 alpha-linolenic and linoleic acids, which are typical substrates of lipoxygenases in flowering plants. To identify the fatty acid substrates used by moss lipoxygenases, eight lipoxygenase genes from Physcomitrella patens were heterologously expressed in Escherichia coli, and then analyzed for lipoxygenase activity using linoleic, alpha-linolenic and arachidonic acids as substrates. Among the eight moss lipoxygenases, only seven were found to be enzymatically active in vitro, two of which selectively used arachidonic acid as the substrate, while the other five preferred alpha-linolenic acid. Based on enzyme assays using a Clark-type oxygen electrode, all of the active lipoxygenases had an optimum pH at 7.0, except for one with highest activity at pH 5.0. HPLC analyses indicated that the two arachidonic acid lipoxygenases form (12S)-hydroperoxy eicosatetraenoic acid as the main product, while the other five lipoxygenases produce mainly (13S)-hydroperoxy octadecatrienoic acid from alpha-linolenic acid. These results suggest that mosses may have both C20 and C18 based oxylipin pathways.
巻・号 70(1)
ページ 40-52
公開日 2009-1-1
DOI 10.1016/j.phytochem.2008.11.012
PII S0031-9422(08)00584-0
PMID 19131081
MeSH Amino Acid Sequence Bryopsida / enzymology* Bryopsida / genetics Eicosanoids / chemistry Eicosanoids / metabolism* Gene Expression Regulation, Plant / physiology Hydrogen-Ion Concentration Lipoxygenase / genetics Lipoxygenase / metabolism* Molecular Sequence Data Phylogeny Stearic Acids / chemistry Stearic Acids / metabolism*
IF 3.044
引用数 34
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdp15520 pdp22648 pdp15606 pdp05498 pdp05507 pdp44232 pdp41344 pdp31865 pdp15933 pdp15246