RRC ID 17670
著者 Tsunashima Y, Kondo A, Matsuda T, Togari A.
タイトル Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts.
ジャーナル Biol Pharm Bull
Abstract Glucocorticoids have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing their proliferation. Using human osteoblastic SaM-1 cells, we examined whether the effects of hydrocortisone on cellular proliferation are mediated by hepatocyte growth factor (HGF). Human osteoblasts constitutively express both HGF and c-Met, its receptor. Hydrocortisone decreased the gene and protein expression of HGF as well as proliferation in SaM-1 cells. These hydrocortisone (0.01-1 µM)-induced decreases in HGF synthesis and cellular proliferation occurred in a concentration-dependent manner. However, no hydrocortisone (0.01-1 µM)-induced decrease in cellular proliferation was observed in human osteosarcoma-derived cells (HOS and SaOS-2), which are not able to produce HGF. In the cellular proliferation in SaM-1 cells, the decrease was blocked concentration-dependently by exogenously applied HGF (0.01-3 ng/ml). Furthermore, SU11274 (1 µM), a highly specific inhibitor of c-Met, suppressed the proliferation of SaM-1 cells, but not HOS cells. From these results, we concluded that hydrocortisone inhibits the proliferation of SaM-1 cells by interrupting the autocrine/paracrine loop via the downregulation of HGF synthesis.
巻・号 34(5)
ページ 700-3
公開日 2011-1-1
DOI 10.1248/bpb.34.700
PII JST.JSTAGE/bpb/34.700
PMID 21532160
MeSH Base Sequence Cell Proliferation / drug effects* DNA Primers Down-Regulation / drug effects* Hepatocyte Growth Factor / biosynthesis* Humans Hydrocortisone / pharmacology* Osteoblasts / drug effects* Osteoblasts / metabolism Reverse Transcriptase Polymerase Chain Reaction
IF 1.863
引用数 3
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 HOS(RCB0992) Saos-2(RCB0428)