RRC ID 44186
著者 Hama S, Uenishi S, Yamada A, Ohgita T, Tsuchiya H, Yamashita E, Kogure K.
タイトル Scavenging of hydroxyl radicals in aqueous solution by astaxanthin encapsulated in liposomes.
ジャーナル Biol Pharm Bull
Abstract Astaxanthin (Asx) is known to be a potent quencher of singlet oxygen and an efficient scavenger of superoxide anion. Therefore, Asx would be expected to be a useful antioxidant for the prevention of oxidative stress, a causative factor in severe diseases such as ischemic reperfusion injury. However, it is still unclear whether Asx has scavenging capability against the most potent reactive oxygen species (ROS), hydroxyl radical, because the hydrophobicity of Asx prevents analysis of hydroxyl radical scavenging ability in aqueous solution. In this study, to solve this problem, liposomes containing Asx (Asx-lipo), which could be dispersed in water, were prepared, and the scavenging ability of Asx-lipo for the hydroxyl radical was examined. The liposomal formulation enabled encapsulation of a high concentration of Asx. Asx-lipo gave a dose-dependent reduction of chemiluminescence intensity induced by hydroxyl radical in aqueous solution. Hydroxyl radical scavenging of Asx was more potent than α-tocopherol. The absorbance of Asx in the liposome decreased after reduction of hydroxyl radicals, indicating the direct hydroxyl radical scavenging by Asx. Moreover, Asx-lipo prevented hydroxyl radical-induced cytotoxicity in cultured NIH-3T3 cells. In conclusion, Asx has potent scavenging capability against hydroxyl radicals in aqueous solution, and this paper is the first report regarding hydroxyl radial scavenging by Asx.
巻・号 35(12)
ページ 2238-42
公開日 2012-1-1
DOI 10.1248/bpb.b12-00715
PII DN/JST.JSTAGE/bpb/b12-00715
PMID 23207776
MeSH Animals Capsules Dose-Response Relationship, Drug Free Radical Scavengers / administration & dosage Free Radical Scavengers / pharmacology* Hydrophobic and Hydrophilic Interactions Hydroxyl Radical / metabolism* Liposomes Mice NIH 3T3 Cells Oxidative Stress / drug effects* Xanthophylls / administration & dosage Xanthophylls / pharmacology alpha-Tocopherol / pharmacology
IF 1.863
引用数 32
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 NIH3T3