RRC ID 50062
著者 Cassar M, Sunderhaus E, Wentzell JS, Kuntz S, Strauss R, Kretzschmar D.
タイトル The PKA-C3 catalytic subunit is required in two pairs of interneurons for successful mating of Drosophila.
ジャーナル Sci Rep
Abstract Protein kinase A (PKA) has been shown to play a role in a plethora of cellular processes ranging from development to memory formation. Its activity is mediated by the catalytic subunits whereby many species express several paralogs. Drosophila encodes three catalytic subunits (PKA-C1-3) and whereas PKA-C1 has been well studied, the functions of the other two subunits were unknown. PKA-C3 is the orthologue of mammalian PRKX/Pkare and they are structurally more closely related to each other than to other catalytic subunits within their species. PRKX is expressed in the nervous system in mice but its function is also unknown. We now show that the loss of PKA-C3 in Drosophila causes copulation defects, though the flies are active and show no defects in other courtship behaviours. This phenotype is specifically due to the loss of PKA-C3 because PKA-C1 cannot replace PKA-C3. PKA-C3 is expressed in two pairs of interneurons that send projections to the ventro-lateral protocerebrum and the mushroom bodies and that synapse onto motor neurons in the ventral nerve cord. Rescue experiments show that expression of PKA-C3 in these interneurons is sufficient for copulation, suggesting a role in relaying information from the sensory system to motor neurons to initiate copulation.
巻・号 8(1)
ページ 2458
公開日 2018-2-6
DOI 10.1038/s41598-018-20697-3
PII 10.1038/s41598-018-20697-3
PMID 29410515
PMC PMC5802784
MeSH Animals Cerebrum / enzymology Cerebrum / physiopathology Copulation* Courtship Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / deficiency Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / genetics* Cyclic AMP-Dependent Protein Kinases / genetics Cyclic AMP-Dependent Protein Kinases / metabolism Drosophila Proteins / deficiency Drosophila Proteins / genetics* Drosophila melanogaster / enzymology Drosophila melanogaster / genetics* Gene Expression Regulation Genetic Complementation Test Interneurons / enzymology* Interneurons / pathology Mice Motor Neurons / enzymology Motor Neurons / pathology Mushroom Bodies / enzymology Mushroom Bodies / physiopathology Protein Serine-Threonine Kinases Reproduction Synapses / enzymology* Synapses / pathology Synaptic Transmission
IF 3.998
引用数 3
リソース情報
ショウジョウバエ 6117R-1 6117R-2