RRC ID 52097
著者 Nagasaka R, Gotou Y, Yoshida K, Kanie K, Shimizu K, Honda H, Kato R.
タイトル Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells.
ジャーナル J Biosci Bioeng
Abstract To meet the growing demand for human induced pluripotent stem cells (iPSCs) for various applications, technologies that enable the manufacturing of iPSCs on a large scale should be developed. There are several technological challenges in iPSC manufacturing technology. Image-based cell quality evaluation technology for monitoring iPSC quality in culture enables the manufacture of intact cells for further applications. Although several studies have reported the effectiveness of image-based evaluation of iPSCs, it remains challenging to detect irregularities that may arise using the same processing operations during quality evaluation of automated processing. In this study, we investigated the evaluation performance of image-based cell quality analysis in detecting small differences that can result from human measurement, even when the same protocol is followed. To imitate such culture conditions, by image-analysis guided colony pickup, we changed the proportions of morphologically different subpopulations: "good morphology, regular morphology correlated with undifferentiation marker expression" and "bad morphology, irregular morphology correlated with loss of undifferentiation marker expression". In addition, comprehensive gene-expression and metabolomics analyses were carried out for the same samples to investigate performance differences. Our data shows an example of investigating the usefulness and sensitivity of quality evaluation methods for iPSC quality monitoring.
巻・号 123(5)
ページ 642-650
公開日 2017-5-1
DOI 10.1016/j.jbiosc.2016.12.015
PII S1389-1723(16)30358-9
PMID 28189491
MeSH Biotechnology / methods* Biotechnology / standards* Cell Differentiation Humans Induced Pluripotent Stem Cells / cytology* Metabolomics Microscopy, Fluorescence Microscopy, Phase-Contrast Molecular Imaging* Quality Control* Transcriptome
IF 2.366
引用数 7
リソース情報
ヒト・動物細胞 201B7(HPS0063)