| RRC ID |
1862
|
| 著者 |
Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A.
|
| タイトル |
Birth of mice produced by germ cell nuclear transfer.
|
| ジャーナル |
Genesis
|
| Abstract |
That mammals can be cloned by nuclear transfer indicates that it is possible to reprogram the somatic cell genome to support full development. However, the developmental plasticity of germ cells is difficult to assess because genomic imprinting, which is essential for normal fetal development, is being reset at this stage. The anomalous influence of imprinting is corroborated by the poor development of mouse clones produced from primordial germ cells (PGCs) during imprinting erasure at embryonic day 11.5 or later. However, this can also be interpreted to mean that, unlike somatic cells, the genome of differentiated germ cells cannot be fully reprogrammed. We used younger PGCs (day 10.5) and eventually obtained four full-term fetuses. DNA methylation analyses showed that only embryos exhibiting normal imprinting developed to term. Thus, germ cell differentiation is not an insurmountable barrier to cloning, and imprinting status is more important than the origin of the nucleus donor cell per se as a determinant of developmental plasticity following nuclear transfer.
|
| 巻・号 |
41(2)
|
| ページ |
81-6
|
| 公開日 |
2005-2-1
|
| DOI |
10.1002/gene.20100
|
| PMID |
15712265
|
| MeSH |
Animals
Animals, Newborn
Cell Differentiation
Cloning, Organism / methods*
DNA Methylation
Female
Genomic Imprinting
Germ Cells / cytology
Male
Mice
Mice, Transgenic
Nuclear Transfer Techniques*
Pregnancy
|
| IF |
1.76
|
| 引用数 |
34
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00821 |
