RRC ID 38129
著者 Ichida Y, Utsunomiya Y, Tomikawa J, Nakabayashi K, Sato T, Onodera M.
タイトル Long time-course monitoring of ZFP809-mediated gene silencing in transgene expression driven by promoters containing MLV-derived PBS.
ジャーナル Biosci Biotechnol Biochem
Abstract Expression of Moloney murine leukemia virus (MoMLV)-typed retroviral vectors is strictly suppressed in immature cells such as embryonic stem cells. The phenomenon known as gene silencing is primed by the sequence-specific binding of the zinc finger protein 809 (ZFP809) to the primer-binding site of the vectors. However, it has yet to be determined whether the ZFP809-mediated gene silencing is maintained over a long period. In this study, we established an experimental system that can monitor gene silencing during a long-term cell culture using flow cytometry technology combined with fluorescent reporters for the expression of ZFP809 and the transgene expression driven by the promoters of interest. Time-course analysis using our system revealed that ZFP809 maintains gene silencing effect even at a longtime period. Furthermore, our system was useful for the monitoring of ZFP809-mediated gene silencing regardless of the types of vectors and cell lines.
巻・号 80(1)
ページ 114-20
公開日 2016-1-1
DOI 10.1080/09168451.2015.1072461
PMID 26252886
MeSH Animals Binding Sites Biological Assay* Cell Line, Tumor DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Gene Silencing* Genes, Reporter Genetic Vectors / chemistry Genetic Vectors / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans K562 Cells Mice Moloney murine leukemia virus / chemistry Moloney murine leukemia virus / genetics Moloney murine leukemia virus / metabolism* Promoter Regions, Genetic Protein Binding Stem Cells / metabolism* Stem Cells / pathology Time Factors
IF 1.516
引用数 1
リソース情報
ヒト・動物細胞 F9