RRC ID 40000
著者 Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC.
タイトル Recoverin depletion accelerates cone photoresponse recovery.
ジャーナル Open Biol
Abstract The neuronal Ca(2+)-binding protein Recoverin has been shown to regulate phototransduction termination in mammalian rods. Here we identify four recoverin genes in the zebrafish genome, rcv1a, rcv1b, rcv2a and rcv2b, and investigate their role in modulating the cone phototransduction cascade. While Recoverin-1b is only found in the adult retina, the other Recoverins are expressed throughout development in all four cone types, except Recoverin-1a, which is expressed only in rods and UV cones. Applying a double flash electroretinogram (ERG) paradigm, downregulation of Recoverin-2a or 2b accelerates cone photoresponse recovery, albeit at different light intensities. Exclusive recording from UV cones via spectral ERG reveals that knockdown of Recoverin-1a alone has no effect, but Recoverin-1a/2a double-knockdowns showed an even shorter recovery time than Recoverin-2a-deficient larvae. We also showed that UV cone photoresponse kinetics depend on Recoverin-2a function via cone-specific kinase Grk7a. This is the first in vivo study demonstrating that cone opsin deactivation kinetics determine overall photoresponse shut off kinetics.
巻・号 5(8)
公開日 2015-8-1
DOI 10.1098/rsob.150086
PII rsob.150086
PMID 26246494
PMC PMC4554923
MeSH Animals G-Protein-Coupled Receptor Kinases / metabolism Gene Expression Gene Knockdown Techniques Molecular Sequence Data Organ Specificity / genetics Recoverin / deficiency Recoverin / genetics Recoverin / metabolism* Retinal Cone Photoreceptor Cells / physiology* Retinal Cone Photoreceptor Cells / radiation effects Ultraviolet Rays Zebrafish Zebrafish Proteins
IF 4.931
引用数 11
リソース情報
ゼブラフィッシュ Tg(zfSWS1-5.5A:EGFP)/kj9 Tg(RH2-2:GFP-PAC)/kj4 / tkk004 Tg(zfSWS2-3.5A:EGFP)/kj11 / tkk008