RRC ID |
37012
|
著者 |
Endo H, Yamaguchi M, Tamura T, Nakano Y, Nishikubo N, Yoneda A, Kato K, Kubo M, Kajita S, Katayama Y, Ohtani M, Demura T.
|
タイトル |
Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.
|
巻・号 |
56(2)
|
ページ |
242-54
|
公開日 |
2015-2-1
|
DOI |
10.1093/pcp/pcu134
|
PII |
pcu134
|
PMID |
25265867
|
MeSH |
Arabidopsis / cytology
Arabidopsis / genetics*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Base Sequence
Cell Differentiation*
Enzyme Assays
Gene Expression Regulation, Plant*
Gene Regulatory Networks
Genes, Plant
Luciferases / metabolism
Models, Biological
Molecular Sequence Data
Nucleotide Motifs / genetics
Plants, Genetically Modified
Promoter Regions, Genetic / genetics
Protein Binding
Transcription Factors / genetics
Transcription Factors / metabolism*
Up-Regulation
Xylem / cytology*
Xylem / genetics*
|
IF |
4.062
|
引用数 |
64
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda00396
pda00727
pda01468
pda01727
pda01764
pda02680
pda03176
pda03329
pda03693
pda05451
pda05682
pda06010
pda06113
pda06907
pda06930
pda08970
pda09137
pda09868 |