RRC ID 21483
著者 Jankowitsch F, Schwarz J, Rückert C, Gust B, Szczepanowski R, Blom J, Pelzer S, Kalinowski J, Mack M.
タイトル Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin.
ジャーナル J Bacteriol
Abstract Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5S) and 69 tRNA genes. The 8,616 predicted protein-coding sequences include 32 clusters coding for secondary metabolites, several of which are unique to S. davawensis. The chromosome contains long terminal inverted repeats of 33,255 bp each and atypical telomeres. Sequence analysis with regard to riboflavin biosynthesis revealed three different patterns of gene organization in Streptomyces species. Heterologous expression of a set of genes present on a subgenomic fragment of S. davawensis resulted in the production of roseoflavin by the host Streptomyces coelicolor M1152. Phylogenetic analysis revealed that S. davawensis is a close relative of Streptomyces cinnabarinus, and much to our surprise, we found that the latter bacterium is a roseoflavin producer as well.
巻・号 194(24)
ページ 6818-27
公開日 2012-12-1
DOI 10.1128/JB.01592-12
PII JB.01592-12
PMID 23043000
PMC PMC3510588
MeSH Anti-Bacterial Agents / biosynthesis* Base Sequence Genome, Bacterial* Multigene Family Phylogeny Plasmids / genetics Riboflavin / analogs & derivatives Riboflavin / biosynthesis Sequence Analysis, DNA Streptomyces / classification Streptomyces / genetics* Streptomyces / metabolism
IF 3.006
引用数 31
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 4913