RRC ID 45787
著者 Li T, Kelly WG.
タイトル A role for WDR5 in TRA-1/Gli mediated transcriptional control of the sperm/oocyte switch in C. elegans.
ジャーナル Nucleic Acids Res
Abstract The hermaphrodite germline of Caenorhabditis elegans initially engages in spermatogenesis and then switches to oogenesis during late stages of larval development. TRA-1, a member of the Ci/Gli family of transcriptional repressors, plays an essential role in this switch by repressing genes that promote spermatogenesis. WDR5 proteins are conserved components of histone methyltransferase complexes normally associated with gene activation. However, two C. elegans WDR5 homologs, wdr-5.1 and wdr-5.2 are redundantly required for normal TRA-1 dependent repression, and this function is independent of their roles in histone methylation. Animals lacking wdr-5.1/wdr-5.2 function fail to switch to oogenesis at 25°C, resulting in a masculinization of germline (Mog) phenotype. The Mog phenotype is caused by ectopic expression of fog-3, a direct target of TRA-1 repression. WDR-5.1 associates with the fog-3 promoter and is required for TRA-1 to bind to fog-3 promoter. Other direct targets of TRA-1 are similarly derepressed in the double mutant. These results show that WDR5 plays a novel and important role in stabilizing transcriptional repression during C. elegans sex determination, and provide evidence that this important protein may operate independently of its established role in histone methyltransferase complexes.
巻・号 42(9)
ページ 5567-81
公開日 2014-5-1
DOI 10.1093/nar/gku221
PII gku221
PMID 24682813
PMC PMC4027197
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA-Binding Proteins / physiology* Epigenesis, Genetic Gene Expression Regulation, Developmental Gene Knockout Techniques Histones / metabolism Male Methylation Oocytes / physiology Oogenesis Protein Processing, Post-Translational Sex Determination Processes Spermatogenesis Spermatozoa / physiology Transcription Factors / physiology* Transcription, Genetic*
IF 11.502
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm1630 tm3463