RRC ID 52016
著者 Nishihara M, Kanda GN, Suzuki T, Yamakado S, Harashima H, Kamiya H.
タイトル Enhanced transgene expression by plasmid-specific recruitment of histone acetyltransferase.
ジャーナル J Biosci Bioeng
Abstract Histone acetylation is associated with the activation of genes on chromosomes. Transgene expression from plasmid DNA might be increased by the acetylation of histones bound to plasmid DNA. To examine this hypothesis, we employed a positive feedback system, using a fusion protein of the sequence-specific DNA binding domain of yeast GAL4 and the histone acetyltransferase (HAT) domain of mouse CREB-binding protein (GAL4-HAT), in which GAL4-HAT promotes its own expression as well as that of a reporter gene product (luciferase). The activator plasmid DNA carrying the gene encoding GAL4-HAT was introduced into mouse Hepa1-6 cells, together with the reporter plasmid DNA, by lipofection. Significantly increased luciferase expression was observed by the co-introduction of the activator plasmid DNA. Moreover, the acetylation of histones bound to the reporter plasmid DNA was enriched by the activator plasmid DNA. These results indicated that the GAL4-HAT system is useful for enhanced transgene expression.
巻・号 123(3)
ページ 277-280
公開日 2017-3-1
DOI 10.1016/j.jbiosc.2016.09.008
PII S1389-1723(16)30293-6
PMID 27746157
MeSH Acetylation Animals CREB-Binding Protein / chemistry Cell Line DNA-Binding Proteins / chemistry Feedback, Physiological Genes, Reporter / genetics Histone Acetyltransferases / chemistry Histone Acetyltransferases / metabolism* Histones / chemistry Histones / metabolism Luciferases / genetics Mice Plasmids / genetics* Protein Domains Protein Processing, Post-Translational Saccharomyces cerevisiae Proteins / chemistry Transcription Factors / chemistry Transfection Transgenes / genetics* Up-Regulation*
IF 2.366
引用数 5
リソース情報
ヒト・動物細胞 Hepa 1-6(RCB1638)