RRC ID 46999
著者 Zhao Y, Cocco C, Domenichini S, Samson ML, Rabinow L.
タイトル The IMD innate immunity pathway of Drosophila influences somatic sex determination via regulation of the Doa locus.
ジャーナル Dev Biol
Abstract The IMD pathway induces the innate immune response to infection by gram-negative bacteria. We demonstrate strong female-to-male sex transformations in double mutants of the IMD pathway in combination with Doa alleles. Doa encodes a protein kinase playing a central role in somatic sex determination through its regulation of alternative splicing of dsx transcripts. Transcripts encoding two specific Doa isoforms are reduced in Rel null mutant females, supporting our genetic observations. A role for the IMD pathway in somatic sex determination is further supported by the induction of female-to-male sex transformations by Dredd mutations in sensitized genetic backgrounds. In contrast, mutations in either dorsal or Dif, the two other NF-κB paralogues of Drosophila, display no effects on sex determination, demonstrating the specificity of IMD signaling. Our results reveal a novel role for the innate immune IMD signaling pathway in the regulation of somatic sex determination in addition to its role in response to microbial infection, demonstrating its effects on alternative splicing through induction of a crucial protein kinase.
巻・号 407(2)
ページ 224-31
公開日 2015-11-15
DOI 10.1016/j.ydbio.2015.09.013
PII S0012-1606(15)30207-4
PMID 26434917
MeSH Alleles Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / immunology* Drosophila melanogaster / ultrastructure Epistasis, Genetic Female Gene Expression Regulation Genes, Insect Genetic Loci* Heterozygote Immunity, Innate / genetics* Male Mutation / genetics NF-kappa B / metabolism Phenotype Promoter Regions, Genetic / genetics Protein Binding Protein Isoforms / genetics Protein Isoforms / metabolism Protein Serine-Threonine Kinases / genetics* Sex Determination Processes / genetics* Signal Transduction / genetics Transcription, Genetic
IF 2.896
引用数 2
WOS 分野 DEVELOPMENTAL BIOLOGY
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