RRC ID 45204
著者 Kudo Y, Abe A, Ito K, Cho Y, Yotsu-Yamashita M, Konoki K.
タイトル Expression of recombinant alpha and beta tubulins from the yew Taxus cuspidata and analysis of the microtubule assembly in the presence of taxol.
ジャーナル Biosci Biotechnol Biochem
Abstract Taxol was originally isolated from the yew Taxus brevifolia. Because taxol inhibits the depolymerization of microtubules, the presence of a self-resistance mechanism in Taxus spp. was hypothesized. The cloning of the cDNA for alpha and beta tubulins from Taxus cuspidata and those from the human embryonic kidney cell line HEK293T revealed that the (26)Asp, (359)Arg, and (361)Leu residues in the human beta tubulin, which are important for taxol binding, were replaced with Glu, Trp, and Met in the beta tubulin of T. cuspidata, respectively. The microtubule assembly of the recombinant alpha and beta tubulins was monitored turbidimetrically, and the results clearly demonstrated that the microtubule from T. cuspidata is less sensitive to taxol than that from HEK293T cells. The Taxus microtubule composed of the wild-type alpha tubulin and the beta tubulin with the E26D mutation restored the sensitivity to taxol. We thus postulated that the mutation identified in the beta tubulin of T. cuspidata plays a role in the self-resistance of this species against taxol.
巻・号 78(11)
ページ 1887-94
公開日 2014-1-1
DOI 10.1080/09168451.2014.940837
PMID 25070196
MeSH Amino Acid Substitution Cloning, Molecular Consensus Sequence Gene Expression Regulation, Plant* HEK293 Cells Humans Microtubules / drug effects* Microtubules / metabolism* Molecular Structure Paclitaxel / pharmacology* Protein Binding / drug effects Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment Taxus / chemistry* Taxus / drug effects Taxus / genetics* Taxus / metabolism Tubulin / chemistry Tubulin / genetics* Tubulin / metabolism Tubulin Modulators / pharmacology
IF 1.516
引用数 3
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 293T(RCB2202)