論文 - 詳細
| RRC ID | 52848 |
|---|---|
| 著者 | Iwata Y, Hayashi N, Tabara K, Mishiba K, Koizumi N. |
| タイトル | Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The N-glycosylation inhibitor tunicamycin triggers endoplasmic reticulum stress response and inhibits efficient protein secretion in eukaryotes. Using Arabidopsis suspension cells, we showed that the reduced secretion of mannose-binding lectin 1 (MBL1) protein by tunicamycin is accompanied by a significant decrease in MBL1 mRNA, suggesting that mRNA destabilization is the major cause of the inhibition of protein secretion in plants. |
| 巻・号 | 80(6) |
| ページ | 1168-71 |
| 公開日 | 2016-6-1 |
| DOI | 10.1080/09168451.2016.1151340 |
| PMID | 26923805 |
| MeSH | Anti-Bacterial Agents / pharmacology* Arabidopsis / drug effects* Arabidopsis / genetics Arabidopsis / metabolism Cell Culture Techniques Culture Media Endoplasmic Reticulum / drug effects* Endoplasmic Reticulum / metabolism Endoplasmic Reticulum Stress / genetics Gene Expression Glycosylation Mannose-Binding Lectin / antagonists & inhibitors* Mannose-Binding Lectin / genetics Mannose-Binding Lectin / metabolism Plant Cells / drug effects* Plant Cells / metabolism RNA Stability RNA, Messenger / metabolism* Tunicamycin / pharmacology* |
| IF | 1.297 |
| 引用数 | 4 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |