RRC ID 49715
著者 Ando K, Fukuhara S, Izumi N, Nakajima H, Fukui H, Kelsh RN, Mochizuki N.
タイトル Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish.
ジャーナル Development
Abstract Mural cells (MCs) consisting of vascular smooth muscle cells and pericytes cover the endothelial cells (ECs) to regulate vascular stability and homeostasis. Here, we clarified the mechanism by which MCs develop and cover ECs by generating transgenic zebrafish lines that allow live imaging of MCs and by lineage tracing in vivo To cover cranial vessels, MCs derived from either neural crest cells or mesoderm emerged around the preformed EC tubes, proliferated and migrated along EC tubes. During their migration, the MCs moved forward by extending their processes along the inter-EC junctions, suggesting a role for inter-EC junctions as a scaffold for MC migration. In the trunk vasculature, MCs derived from mesoderm covered the ventral side of the dorsal aorta (DA), but not the posterior cardinal vein. Furthermore, the MCs migrating from the DA or emerging around intersegmental vessels (ISVs) preferentially covered arterial ISVs rather than venous ISVs, indicating that MCs mostly cover arteries during vascular development. Thus, live imaging and lineage tracing enabled us to clarify precisely how MCs cover the EC tubes and to identify the origins of MCs.
巻・号 143(8)
ページ 1328-39
公開日 2016-4-15
DOI 10.1242/dev.132654
PII dev.132654
PMID 26952986
PMC PMC4852519
MeSH Animals Animals, Genetically Modified Blood Vessels / cytology Blood Vessels / embryology Endothelial Cells / cytology* Microscopy, Confocal Muscle, Smooth, Vascular / cytology* Pericytes / cytology* Zebrafish
IF 5.611
引用数 56
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