RRC ID 32413
著者 Qian Y, Ng CL, Schulz C.
タイトル CSN maintains the germline cellular microenvironment and controls the level of stem cell genes via distinct CRLs in testes of Drosophila melanogaster.
ジャーナル Dev Biol
Abstract Stem cells and their daughters are often associated with and depend on cues from their cellular microenvironment. In Drosophila testes, each Germline Stem Cell (GSC) contacts apical hub cells and is enclosed by cytoplasmic extensions from two Cyst Stem Cells (CySCs). Each GSC daughter becomes enclosed by cytoplasmic extensions from two CySC daughters, called cyst cells. CySC fate depends on an Unpaired (Upd) signal from the hub cells, which activates the Janus Kinase and Signal Transducer and Activator of Transcription (Jak/STAT) pathway in the stem cells. Germline enclosure depends on Epidermal Growth Factor (EGF) signals from the germline to the somatic support cells. Expression of RNA-hairpins against subunits of the COnstitutively Photomorphogenic-9- (COP9-) signalosome (CSN) in somatic support cells disrupted germline enclosure. Furthermore, CSN-depleted somatic support cells in the CySC position next to the hub had reduced levels of the Jak/STAT effectors Zinc finger homeotic-1 (Zfh-1) and Chronologically inappropriate morphogenesis (Chinmo). Knockdown of CSN in the somatic support cells does not disrupt EGF and Upd signal transduction as downstream signal transducers, phosphorylated STAT (pSTAT) and phosphorylated Mitogen Activated Protein Kinase (pMAPK), were still localized to the somatic support cell nuclei. The CSN modifies fully formed Cullin RING ubiquitin ligase (CRL) complexes to regulate selective proteolysis. Reducing cullin2 (cul2) from the somatic support cells disrupted germline enclosure, while reducing cullin1 (cul1) from the somatic support cells led to a low level of Chinmo. We propose that different CRLs enable the responses of somatic support cells to Upd and EGF.
巻・号 398(1)
ページ 68-79
公開日 2015-2-1
DOI 10.1016/j.ydbio.2014.11.014
PII S0012-1606(14)00591-0
PMID 25459658
MeSH Animals Cell Lineage Crosses, Genetic Cullin Proteins / metabolism Cytoplasm / metabolism Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / embryology Germ Cells / physiology Janus Kinases / metabolism MAP Kinase Signaling System Male Microscopy, Fluorescence Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / physiology* Phenotype RNA / analysis RNA Interference Repressor Proteins / genetics Repressor Proteins / physiology* STAT Transcription Factors / metabolism Signal Transduction Stem Cells / cytology* Testis / embryology* Testis / pathology*
IF 2.896
引用数 15
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ tj-Gal4