RRC ID 33576
著者 Tojo S, Hirooka K, Fujita Y.
タイトル Expression of kinA and kinB of Bacillus subtilis, necessary for sporulation initiation, is under positive stringent transcription control.
ジャーナル J Bacteriol
Abstract Bacillus subtilis cells were exposed to decoyinine to trigger stringent transcription control through inhibition of GMP synthase; amino acid starvation results in the same control through inhibition of GMP kinase by 5'-diphosphate 3'-diphosphate guanosine. The positive and negative transcription control of the stringent genes involves adenine and guanine at the transcription initiation sites, whereby they sense an increase and a decrease in the in vivo ATP and GTP pools, respectively. Decoyinine also induces sporulation in minimum medium. DNA microarray analysis revealed that decoyinine induced two major sensor kinase genes, kinA and kinB, involved in the phosphorelay leading to spore formation. lacZ fusion experiments involving the core promoter regions of kinA and kinB, whose transcription initiation bases are adenines, indicated that decoyinine induced their expression. This induction was independent of CodY and AbrB. When the adenines were replaced with guanines or cytosines, the induction by decoyinine decreased. The in situ replacement of the adenines with guanines actually affected this decoyinine-induced sporulation as well as massive sporulation in nutrient medium. These results imply that operation of the positive stringent transcription control of kinA and kinB, which is mediated by an increase in the ATP pool, is likely a prerequisite for the phosphorelay to transfer the phosphoryl group to Spo0A to initiate sporulation.
巻・号 195(8)
ページ 1656-65
公開日 2013-4-1
DOI 10.1128/JB.02131-12
PII JB.02131-12
PMID 23378509
PMC PMC3624560
MeSH Bacillus subtilis / metabolism Bacillus subtilis / physiology* Bacterial Proteins / genetics Bacterial Proteins / metabolism* DNA, Bacterial / genetics DNA, Bacterial / metabolism Down-Regulation Gene Expression Regulation, Bacterial / physiology* Guanosine Triphosphate / metabolism Oligonucleotide Array Sequence Analysis Phosphates / metabolism Phosphotransferases / genetics Phosphotransferases / metabolism* Protein Kinases / genetics Protein Kinases / metabolism* Spores, Bacterial / genetics Spores, Bacterial / physiology Transcription, Genetic / physiology*
IF 3.006
引用数 16
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS340 MBS341 MBS342 MBS343 MBS344 MBS345 MBS346 MBS347 MBS348 MBS349 MBS350 MBS351 MBS352 MBS353 MBS354 MBS355 MBS371 MBS372 MBS373 MBS374