RRC ID 47758
著者 Hao S, Ariizumi T, Ezura H.
タイトル SEXUAL STERILITY is Essential for Both Male and Female Gametogenesis in Tomato.
ジャーナル Plant Cell Physiol
Abstract Gametogenesis is a key step in the production of ovules or pollen in higher plants. The molecular aspects of gametogenesis are well characterized in the model plant Arabidopsis; however, little information is known in tomato, which is a model plant for fleshy fruit development. In this study, we characterized a tomato (Solanum lycopersicum L.) γ-ray mutant, sexual sterility (Slses), that exhibited both male and female sterility. Morphological analysis revealed that the Slses mutant forms incomplete ovules and wilted anthers devoid of pollen grains at the anthesis stage. Genetic and next-generation sequencing analyses revealed that the Slses mutant carried a 13 bp deletion within the first exon of a homolog of SPOROCYTELESS/NOZZLE (SPL/NZZ), which plays an important role in gametogenesis in Arabidopsis. Complementation analysis in which the complete SlSES genomic region was introduced into the Slses mutant fully restored normal phenotypes, demonstrating that Solyc07g063670 is responsible for the Slses mutation. SlSES probably act as a transcriptional repressor because of an EAR motif at the C-terminal region. Gene expression levels of WUSCHEL (SlWUS) and INNER NO OUTER (SlINO), both of which are required for ovule development, were dramatically reduced in the early stages of pistil development in the Slses mutant, suggesting a positive regulatory role for SlSES in the transcription of gametogenesis genes and differences in the regulation of INO (SlINO) and integument development by SPL/NZZ (SLSES) between Arabidopsis and tomato. Taken together, our results indicate that SlSES is a novel tomato gametogenesis gene essential for both male and female gametogenesis.
巻・号 58(1)
ページ 22-34
公開日 2017-1-1
DOI 10.1093/pcp/pcw214
PII pcw214
PMID 28082517
MeSH Amino Acid Sequence Base Sequence Flowers / genetics Flowers / growth & development Gene Deletion Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Models, Genetic Mutation* Ovule / genetics* Ovule / growth & development Phenotype Phylogeny Plant Infertility / genetics* Plant Proteins / classification Plant Proteins / genetics Plants, Genetically Modified Pollen / genetics* Pollen / growth & development Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Solanum lycopersicum / genetics* Solanum lycopersicum / growth & development Solanum lycopersicum / radiation effects
IF 4.062
引用数 13
WOS 分野 CELL BIOLOGY PLANT SCIENCES
リソース情報
トマト TOMJPF00001 TOMJPF00004