RRC ID 34877
著者 Momiuchi Y, Kumada K, Kuraishi T, Takagaki T, Aigaki T, Oshima Y, Kurata S.
タイトル The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-κB Signaling.
ジャーナル J Biol Chem
Abstract The NF-κB pathway is a phylogenetically conserved signaling pathway with a central role in inflammatory and immune responses. Here we demonstrate that a cochaperone protein, Droj2/DNAJA3, is involved in the activation of canonical NF-κB signaling in flies and in human cultured cells. Overexpression of Droj2 induced the expression of an antimicrobial peptide in Drosophila. Conversely, Droj2 knockdown resulted in reduced expression of antimicrobial peptides and higher susceptibility to Gram-negative bacterial infection in flies. Similarly, Toll-like receptor-stimulated IκB phosphorylation and NF-κB activation were suppressed by DNAJA3 knockdown in HEK293 cells. IκB kinase overexpression-induced NF-κB phosphorylation was also compromised in DNAJA3 knockdown cells. Our study reveals a novel conserved regulator of the NF-κB pathway acting at the level of IκB phosphorylation.
巻・号 290(39)
ページ 23816-25
公開日 2015-9-25
DOI 10.1074/jbc.M115.664193
PII S0021-9258(20)44719-2
PMID 26245905
PMC PMC4583039
MeSH Animals Antimicrobial Cationic Peptides / biosynthesis Antimicrobial Cationic Peptides / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Gene Knockdown Techniques HEK293 Cells HSP40 Heat-Shock Proteins / biosynthesis* HSP40 Heat-Shock Proteins / genetics Humans I-kappa B Kinase / genetics I-kappa B Kinase / metabolism NF-kappa B / genetics NF-kappa B / metabolism* Phosphorylation / physiology Phylogeny Signal Transduction / physiology*
IF 4.238
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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