論文 - 詳細
RRC ID | 50318 |
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著者 | Schartl M, Schories S, Wakamatsu Y, Nagao Y, Hashimoto H, Bertin C, Mourot B, Schmidt C, Wilhelm D, Centanin L, Guiguen Y, Herpin A. |
タイトル | Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements. |
ジャーナル | BMC Biol |
Abstract |
BACKGROUND:Sex determination relies on a hierarchically structured network of genes, and is one of the most plastic processes in evolution. The evolution of sex-determining genes within a network, by neo- or sub-functionalization, also requires the regulatory landscape to be rewired to accommodate these novel gene functions. We previously showed that in medaka fish, the regulatory landscape of the master male-determining gene dmrt1bY underwent a profound rearrangement, concomitantly with acquiring a dominant position within the sex-determining network. This rewiring was brought about by the exaptation of a transposable element (TE) called Izanagi, which is co-opted to act as a silencer to turn off the dmrt1bY gene after it performed its function in sex determination. RESULTS:We now show that a second TE, Rex1, has been incorporated into Izanagi. The insertion of Rex1 brought in a preformed regulatory element for the transcription factor Sox5, which here functions in establishing the temporal and cell-type-specific expression pattern of dmrt1bY. Mutant analysis demonstrates the importance of Sox5 in the gonadal development of medaka, and possibly in mice, in a dmrt1bY-independent manner. Moreover, Sox5 medaka mutants have complete female-to-male sex reversal. CONCLUSIONS:Our work reveals an unexpected complexity in TE-mediated transcriptional rewiring, with the exaptation of a second TE into a network already rewired by a TE. We also show a dual role for Sox5 during sex determination: first, as an evolutionarily conserved regulator of germ-cell number in medaka, and second, by de novo regulation of dmrt1 transcriptional activity during primary sex determination due to exaptation of the Rex1 transposable element. |
巻・号 | 16(1) |
ページ | 16 |
公開日 | 2018-1-29 |
DOI | 10.1186/s12915-018-0485-8 |
PII | 10.1186/s12915-018-0485-8 |
PMID | 29378592 |
PMC | PMC5789577 |
MeSH | Animals Animals, Genetically Modified DNA Transposable Elements / physiology* Female Germ Cells / metabolism* Male Oryzias SOXD Transcription Factors / biosynthesis* SOXD Transcription Factors / genetics Sex Chromosomes / genetics Sex Chromosomes / metabolism* Sex Determination Processes / physiology* |
IF | 6.765 |
引用数 | 9 |
リソース情報 | |
メダカ | sox5^N541S (MT1048) |