RRC ID 40794
著者 Mitsui R, Katayama H, Tanaka M.
タイトル Requirement of carbon dioxide for initial growth of facultative methylotroph, Acidomonas methanolica MB58.
ジャーナル J Biosci Bioeng
Abstract The facultative methylotrophic bacterium Acidomonas methanolica MB58 can utilize C1 compounds via the ribulose monophosphate pathway. A large gene cluster comprising three components related to C1 metabolism was found in the genome. From upstream, the first was an mxa cluster encoding proteins for oxidation of methanol to formaldehyde; the second was the rmp cluster encoding enzymes for formaldehyde fixation; and the third was the cbb gene cluster encoding proteins for carbon dioxide (CO2) fixation. Examination of CO2 requirements for growth of A. methanolica MB58 cells demonstrated that it did not grow on any carbon source under CO2-free conditions. Measurement of ribulose-1,5-bisphosphate carboxylase activity and RT-PCR analysis demonstrated enzymatic activity was detected in A. methanolica MB58 at growth phase, regardless of carbon sources. However, methanol dehydrogenase and 3-hexlose-6-phosphate synthase expression was regulated by methanol or formaldehyde; it were detected during growth and apparently differed from ribulose-1,5-bisphosphate carboxylase expression. These results suggested that A. methanolica MB58 may be initially dependent on autotrophic growth and that carbon assimilation was subsequently coupled with the ribulose monophosphate pathway at early- to mid-log phases during methylotrophic growth.
巻・号 120(1)
ページ 31-5
公開日 2015-7-1
DOI 10.1016/j.jbiosc.2014.11.016
PII S1389-1723(14)00455-1
PMID 25511787
MeSH Acetobacteraceae / genetics Acetobacteraceae / growth & development* Acetobacteraceae / metabolism* Alcohol Oxidoreductases / metabolism Carbon / metabolism Carbon Dioxide / metabolism* Formaldehyde / metabolism Genes, Bacterial / genetics Methanol / metabolism Multigene Family / genetics Oxidation-Reduction Photosynthesis Ribulose-Bisphosphate Carboxylase / metabolism
IF 2.366
引用数 1
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY FOOD SCIENCE & TECHNOLOGY
リソース情報
一般微生物 JCM 6891