論文 - 詳細
RRC ID | 38879 |
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著者 | Jin Y, Tsuchiya A, Kanno T, Nishizaki T. |
タイトル | Amyloid-β peptide increases cell surface localization of α7 ACh receptor to protect neurons from amyloid β-induced damage. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
Amyloid-β peptide 1-42 (Aβ1-42) reduced PC-12 cell viability in a concentration (1-10 μM)- and treatment time (48-72 h)-dependent manner. Nicotine prevented Aβ1-42-induced PC-12 cell death, but conversely, the α7 ACh receptor antagonist α-bungarotoxin enhanced Aβ1-42-induced cell toxicity. Extracellularly applied Aβ1-42 significantly increased cell surface localization of α7 ACh receptor in PC-12 cells as compared with that for non-treated control cells. Cell surface localization of α7 ACh receptor in the brain of 5xFAD mouse, an animal model of Alzheimer's disease (AD), apparently increased in an age (1-12 months)-dependent manner in association with increased accumulation of Aβ1-42 in the plasma membrane component. Taken together, these results indicate that Aβ1-42 promotes translocation of α7 ACh receptor towards the cell surface and that α7 ACh receptor rescues neuronal cells from Aβ1-42-induced damage. |
巻・号 | 468(1-2) |
ページ | 157-60 |
公開日 | 2015-12-4 |
DOI | 10.1016/j.bbrc.2015.10.141 |
PII | S0006-291X(15)30838-X |
PMID | 26522221 |
MeSH | Alzheimer Disease / drug therapy Alzheimer Disease / metabolism Alzheimer Disease / pathology Amyloid beta-Peptides / metabolism* Amyloid beta-Peptides / pharmacology* Animals Brain / drug effects Brain / metabolism Brain / pathology Cell Survival / drug effects Mice Neurons / cytology* Neurons / drug effects* Neurons / metabolism Neurons / pathology Neuroprotective Agents / metabolism* Neuroprotective Agents / pharmacology* Nicotine / pharmacology PC12 Cells Peptide Fragments / metabolism* Peptide Fragments / pharmacology* Protein Transport / drug effects Rats alpha7 Nicotinic Acetylcholine Receptor / agonists alpha7 Nicotinic Acetylcholine Receptor / analysis alpha7 Nicotinic Acetylcholine Receptor / metabolism* |
IF | 2.985 |
引用数 | 11 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | PC-12(RCB0009) |