RRC ID 49636
著者 Tan SWS, Yip GW, Suda T, Baeg GH.
タイトル Small Maf functions in the maintenance of germline stem cells in the Drosophila testis.
ジャーナル Redox Biol
Abstract Reactive oxygen species (ROS) are byproducts generated during normal cellular metabolism, and redox states have been shown to influence stem cell self-renewal and lineage commitment across phyla. However, the downstream effectors of ROS signaling that control stem cell behavior remain largely unexplored. Here, we used the Drosophila testis as an in vivo model to identify ROS-induced effectors that are involved in the differentiation process of germline stem cells (GSCs). In the Affymetrix microarray analysis, 152 genes were either upregulated or downregulated during GSC differentiation induced by elevated levels of ROS, and a follow-up validation of the gene expression by qRT-PCR showed a Spearman's rho of 0.9173 (P<0.0001). Notably, 47 (31%) of the identified genes had no predicted molecular function or recognizable protein domain. These suggest the robustness of this microarray analysis, which identified many uncharacterized genes, possibly with an essential role in ROS-induced GSC differentiation. We also showed that maf-S is transcriptionally downregulated by oxidative stress, and that maf-S knockdown promotes GSC differentiation but Maf-S overexpression conversely results in an over-growth of GSC-like cells by promoting the mitotic activity of germ cell lineage. Together with the facts that Maf-S regulates ROS levels and genetically interacts with Keap1/Nrf2 in GSC maintenance, our study suggests that Maf-S plays an important role in the Drosophila testis GSC maintenance by participating in the regulation of redox homeostasis.
巻・号 15
ページ 125-134
公開日 2018-5-1
DOI 10.1016/j.redox.2017.12.002
PII S2213-2317(17)30894-7
PMID 29245136
PMC PMC5730423
MeSH Adult Germline Stem Cells / metabolism Animals Cell Differentiation / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Gene Expression Regulation / genetics Kelch-Like ECH-Associated Protein 1 / genetics* Male NF-E2-Related Factor 2 / genetics* Reactive Oxygen Species / metabolism Repressor Proteins / genetics* Testis / growth & development Testis / metabolism* Tissue Array Analysis
IF 9.986
引用数 11
リソース情報
ショウジョウバエ 2286R-2 2286R-3 6343R-1 6343R-2