RRC ID 41038
著者 Nguyen TM, Pham VH, Kim J.
タイトル Mesorhizobium soli sp. nov., a novel species isolated from the rhizosphere of Robinia pseudoacacia L. in South Korea by using a modified culture method.
ジャーナル Antonie Van Leeuwenhoek
Abstract Strain NHI-8(T) was isolated from a forest soil sample, collected in South Korea, by using a modified culture method. Comparative analysis of its nearly full-length 16S rRNA gene sequence showed that strain NHI-8(T) belongs to the genus Mesorhizobium and to be closely related to Mesorhizobium chacoense PR5(T) (97.32 %). The levels of DNA-DNA relatedness between strain NHI-8(T) and reference type strains of the genus Mesorhizobium were 32.28-53.65 %. SDS-PAGE of total soluble proteins and the sequences of the housekeeping genes recA, glnII, and atpD were also used to support the clade grouping in rhizobia. The new strain contained summed feature 8 (57.0 %), cyclo-C19:0ω8c (17.3 %), and C18:0 (11.0 %) as the major fatty acids, as in genus Mesorhizobium. The strain contained cardiolipin, phosphatidylglycerol, ornithine-containing lipid, phosphatidylethanolamine, phosphatidyl-N-dimethylethanolamine, and phosphatidylcholine. Morphological and physiological analyses were performed to compare the characteristics of our strain with those of the reference type strains. Based on the results, strain NHI-8(T) was determined to represent a novel member of the genus Mesorhizobium, and the name Mesorhizobium soli is proposed. The type strain is NHI-8(T) (=KEMB 9005-153(T) = KACC 17916(T) = JCM 19897(T)).
巻・号 108(2)
ページ 301-10
公開日 2015-8-1
DOI 10.1007/s10482-015-0481-8
PMID 25980835
MeSH Bacterial Proteins / analysis Bacterial Typing Techniques Cell Wall / chemistry Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Electrophoresis, Polyacrylamide Gel Fatty Acids / analysis Forests Genes, Essential Mesorhizobium / classification* Mesorhizobium / genetics Mesorhizobium / isolation & purification* Mesorhizobium / physiology Molecular Sequence Data Nucleic Acid Hybridization Ornithine / analysis Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Republic of Korea Rhizosphere* Robinia / growth & development* Sequence Analysis, DNA Soil Microbiology*
IF 2.674
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19897