RRC ID 41064
著者 Zhang WY, Hu J, Zhang XQ, Zhu XF, Wu M.
タイトル Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel aerobic bacterium, KC1(T), was isolated from a salt mine in Kuche county, Xinjiang province, China. Cells were observed to be Gram-positive, rod-shaped, endospore-forming and motile with flagella. Strain KC1(T) was found to grow at 25-45 °C (optimum 37 °C), pH 6.5-9.0 (optimum 8.0) and NaCl 0-10 % (v/v) (optimum 4 %). The major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. Menaquinone-7 (MK-7) was found to be the predominant isoprenoid quinone. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol and a glycolipid. The 16S rRNA gene sequence of strain KC1(T) showed low similarity (<96 %) to other validly named species. The phylogenetic trees showed that strain KC1(T) is closely related to Bacillus azotoformans DSM 1046(T) and Bacillus methanolicus DSM 16454(T). Both these type strains showed 95.4 % 16S rRNA gene sequence similarity to strain KC1(T). The DNA G+C content of strain KC1(T) was determined to be 39.0 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KC1(T) is considered to represent a novel species of the genus Bacillus, for which the name Bacillus salitolerans sp. nov. is proposed. The type strain is KC1(T) (=JCM 19760(T) = CGMCC 1.12810(T)).
巻・号 108(2)
ページ 443-51
公開日 2015-8-1
DOI 10.1007/s10482-015-0497-0
PMID 26076748
MeSH Aerobiosis Bacillus / classification* Bacillus / genetics Bacillus / isolation & purification* Bacillus / physiology Base Composition Cell Wall / chemistry China Cluster Analysis Cytosol / chemistry DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Environmental Microbiology* Fatty Acids / analysis Hydrogen-Ion Concentration Locomotion Molecular Sequence Data Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Salts Sequence Analysis, DNA Sodium Chloride / metabolism Temperature Vitamin K 2 / analysis
IF 2.674
引用数 2
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19760