RRC ID 41070
著者 Zhang MY, Xu H, Zhang TY, Xie J, Cheng J, Nimaichand S, Li SH, Li WJ, Zhang YX.
タイトル Flavobacterium notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng.
ジャーナル Antonie Van Leeuwenhoek
Abstract Two novel bacterial strains, designated SYP-B540(T) and SYP-B556, were isolated from rhizospheric soil of Panax notoginseng located at Yunnan Province, China. Both strains were Gram-staining negative, aerobic, non-motile, elongated rod shaped and yellow coloured. They grew optimally at 28 °C and pH 7.0. Analysis of 16S rRNA gene sequences showed that the two strains shared 99.8 % sequence similarity to each other, but lower than 97.6 % to the other known species of the genus Flavobacterium. The predominant respiratory quinone for the two strains was MK-6, and the major fatty acids were iso-C15:0 and summed Feature 3 (comprising 16:1 ω7c and/or 16:1 ω6c). The polar lipids consisted of phosphatidylethanolamine, two unidentified polar lipids and three unidentified amino-phospholipids. The DNA G+C contents of strains SYP-B540(T) and SYP-B556 were 33.3 and 32.7 mol%, respectively. In addition, the DNA-DNA hybridization values of strains SYP-B540(T) and SYP-B556 to their closest phylogenetic neighbors were significantly lower than 70 %. On the basis of the polyphasic taxonomy studies, strains SYP-B540(T) and SYP-B556 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium notoginsengisoli sp. nov. is proposed. The type strain is SYP-B540(T) (=KCTC 32505(T) = NBRC 110012(T) = BCRC 80724(T)).
巻・号 108(3)
ページ 545-52
公開日 2015-9-1
DOI 10.1007/s10482-015-0509-0
PMID 26111850
MeSH Aerobiosis Bacterial Typing Techniques Base Composition China Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Flavobacterium / classification* Flavobacterium / genetics Flavobacterium / isolation & purification* Flavobacterium / physiology Hydrogen-Ion Concentration Microscopy, Electron, Transmission Molecular Sequence Data Nucleic Acid Hybridization Panax notoginseng / growth & development* Phospholipids / analysis Phylogeny Pigments, Biological / metabolism Quinones / analysis RNA, Ribosomal, 16S / genetics Rhizosphere* Sequence Analysis, DNA Soil Microbiology* Temperature
IF 2.674
引用数 7
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 8514