RRC ID 45567
著者 Gomez-Amaro RL, Valentine ER, Carretero M, LeBoeuf SE, Rangaraju S, Broaddus CD, Solis GM, Williamson JR, Petrascheck M.
タイトル Measuring Food Intake and Nutrient Absorption in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Caenorhabditis elegans has emerged as a powerful model to study the genetics of feeding, food-related behaviors, and metabolism. Despite the many advantages of C. elegans as a model organism, direct measurement of its bacterial food intake remains challenging. Here, we describe two complementary methods that measure the food intake of C. elegans. The first method is a microtiter plate-based bacterial clearing assay that measures food intake by quantifying the change in the optical density of bacteria over time. The second method, termed pulse feeding, measures the absorption of food by tracking de novo protein synthesis using a novel metabolic pulse-labeling strategy. Using the bacterial clearance assay, we compare the bacterial food intake of various C. elegans strains and show that long-lived eat mutants eat substantially more than previous estimates. To demonstrate the applicability of the pulse-feeding assay, we compare the assimilation of food for two C. elegans strains in response to serotonin. We show that serotonin-increased feeding leads to increased protein synthesis in a SER-7-dependent manner, including proteins known to promote aging. Protein content in the food has recently emerged as critical factor in determining how food composition affects aging and health. The pulse-feeding assay, by measuring de novo protein synthesis, represents an ideal method to unequivocally establish how the composition of food dictates protein synthesis. In combination, these two assays provide new and powerful tools for C. elegans research to investigate feeding and how food intake affects the proteome and thus the physiology and health of an organism.
巻・号 200(2)
ページ 443-54
公開日 2015-6-1
DOI 10.1534/genetics.115.175851
PII genetics.115.175851
PMID 25903497
PMC PMC4492371
MeSH Animal Feed* Animals Body Size Caenorhabditis elegans / physiology* Feeding Behavior* Mass Spectrometry Mutation Protein Biosynthesis / drug effects Serotonin / metabolism Serotonin / pharmacology
IF 4.015
引用数 33
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm2146 tm1325