RRC ID 50338
著者 Yamamoto T, Hoshikawa K, Ezura K, Okazawa R, Fujita S, Takaoka M, Mason HS, Ezura H, Miura K.
タイトル Improvement of the transient expression system for production of recombinant proteins in plants.
ジャーナル Sci Rep
Abstract An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient expression system can be used to identify the localization of proteins in plant cells. In this study, we demonstrated that combination of a geminiviral replication and a double terminator dramatically enhanced the transient protein expression level in plants. The GFP protein was expressed transiently in lettuce, Nicotiana benthamiana, tomatoes, eggplants, hot peppers, melons, and orchids with agroinfiltration. Compared to a single terminator, a double terminator enhanced the expression level. A heat shock protein terminator combined with an extensin terminator resulted in the highest protein expression. Transiently expressed GFP was confirmed by immunoblot analysis with anti-GFP antibodies. Quantitative analysis revealed that the geminiviral vector with a double terminator resulted in the expression of at least 3.7 mg/g fresh weight of GFP in Nicotiana benthamiana, approximately 2-fold that of the geminiviral vector with a single terminator. These results indicated that combination of the geminiviral replication and a double terminator is a useful tool for transient expression of the gene of interest in plant cells.
巻・号 8(1)
ページ 4755
公開日 2018-3-19
DOI 10.1038/s41598-018-23024-y
PII 10.1038/s41598-018-23024-y
PMID 29555968
PMC PMC5859073
MeSH Gene Expression Genetic Engineering / methods* Genetic Vectors / genetics Plants / genetics* Recombinant Proteins / biosynthesis Recombinant Proteins / genetics*
IF 3.998
引用数 19
リソース情報
トマト TOMJPF00001