RRC ID 37944
著者 Kikuta S, Hou BH, Sato R, Frommer WB, Kikawada T.
タイトル FRET sensor-based quantification of intracellular trehalose in mammalian cells.
ジャーナル Biosci Biotechnol Biochem
Abstract Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIP-suc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.
巻・号 80(1)
ページ 162-5
公開日 2016-1-1
DOI 10.1080/09168451.2015.1069699
PMID 26214383
MeSH Animals Bacterial Proteins / genetics* Bacterial Proteins / metabolism Biosensing Techniques* Carrier Proteins / genetics* Carrier Proteins / metabolism Cytoplasm / drug effects Cytoplasm / metabolism Diptera Escherichia coli / genetics Escherichia coli / metabolism Fluorescence Resonance Energy Transfer / methods* Gene Expression Genetic Vectors / chemistry Genetic Vectors / metabolism* HEK293 Cells Humans Insect Proteins / genetics Insect Proteins / metabolism Luminescent Proteins / genetics* Luminescent Proteins / metabolism Transfection Trehalose / analysis* Trehalose / metabolism Trehalose / pharmacology
IF 1.516
引用数 5
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
ヒト・動物細胞 293T(RCB2202)