論文 - 詳細
RRC ID | 37503 |
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著者 | Motoyama K, Sako A, Ibrahim Abu Hashim I, Higashi T, Arima H. |
タイトル | Effects of dendrimer/cyclodextrin conjugates as gene transfer carriers on nitric oxide production from macrophages. |
ジャーナル | J Pharm Pharmacol |
Abstract |
OBJECTIVES:The development of safe gene transfer carriers with high transfection efficiency, which does not affect the cell function, is a challenging issue. In this study, we examined the effects of α-cyclodextrin (α-CyD)/dendrimer conjugate (α-CDE (G3)) on nitric oxide (NO) production in murine macrophages J774.1 cells stimulated with toll-like receptors (TLR) ligands. METHODS:NO production from macrophages stimulated with TLR ligands was determined by the Griess method. Transfection efficiency of α-CDE (G3)/plasmid DNA (pDNA) complex was quantified by a luminometer. KEY FINDINGS:α-CDE (G3) significantly inhibited NO production from J774.1 cells stimulated with TLR ligands. α-CyD molecules in α-CDE (G3) had no effect on NO production. The inhibitory effect of α-CDE (G3) on NO production might be attributed to the dendrimer (G3). Increasing the degree of substitution (DS) of α-CyD in the α-CDE (G3) molecule was accompanied by a significant decrease in the inhibition of NO production. Furthermore, higher gene transfection efficiency of α-CDE (G3)/pDNA complex was observed upon increasing the DS of α-CyD. CONCLUSIONS:α-CDE (G3) with high DS value of α-CyD may be considered as a safe gene transfer carrier that does not adversely affect NO production from macrophages stimulated with TLR ligands. |
巻・号 | 68(5) |
ページ | 598-607 |
公開日 | 2016-5-1 |
DOI | 10.1111/jphp.12439 |
PMID | 26076676 |
MeSH | Animals Cell Line Cell Survival / drug effects Dendrimers / chemistry Dendrimers / metabolism Dendrimers / toxicity* Dose-Response Relationship, Drug Ligands Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / metabolism* Nitrites / metabolism Toll-Like Receptors / agonists Toll-Like Receptors / metabolism Transfection / methods* alpha-Cyclodextrins / chemistry alpha-Cyclodextrins / metabolism alpha-Cyclodextrins / toxicity* |
IF | 2.571 |
引用数 | 0 |
WOS 分野 | PHARMACOLOGY & PHARMACY |
リソース情報 | |
ヒト・動物細胞 | J774.1(RCB0434) |