RRC ID 37577
著者 Correia V, Panadero JA, Ribeiro C, Sencadas V, Rocha JG, Gomez Ribelles JL, Lanceros-Méndez S.
タイトル Design and validation of a biomechanical bioreactor for cartilage tissue culture.
ジャーナル Biomech Model Mechanobiol
Abstract Specific tissues, such as cartilage, undergo mechanical solicitation under their normal performance in human body. In this sense, it seems necessary that proper tissue engineering strategies of these tissues should incorporate mechanical solicitations during cell culture, in order to properly evaluate the influence of the mechanical stimulus. This work reports on a user-friendly bioreactor suitable for applying controlled mechanical stimulation--amplitude and frequency--to three-dimensional scaffolds. Its design and main components are described, as well as its operation characteristics. The modular design allows easy cleaning and operating under laminar hood. Different protocols for the sterilization of the hermetic enclosure are tested and ensure lack of observable contaminations, complying with the requirements to be used for cell culture. The cell viability study was performed with KUM5 cells.
巻・号 15(2)
ページ 471-8
公開日 2016-4-1
DOI 10.1007/s10237-015-0698-5
PII 10.1007/s10237-015-0698-5
PMID 26153426
MeSH Animals Bioreactors* Cartilage, Articular / growth & development* Cell Line Electricity Equipment Design Mice Reproducibility of Results Software Tissue Engineering / instrumentation* Tissue Engineering / methods*
IF 2.527
引用数 6
WOS 分野 ENGINEERING, BIOMEDICAL BIOPHYSICS
リソース情報
ヒト・動物細胞 KUM5(RCB2322)