RRC ID |
37577
|
著者 |
Correia V, Panadero JA, Ribeiro C, Sencadas V, Rocha JG, Gomez Ribelles JL, Lanceros-Méndez S.
|
タイトル |
Design and validation of a biomechanical bioreactor for cartilage tissue culture.
|
ジャーナル |
Biomech Model Mechanobiol
|
Abstract |
Specific tissues, such as cartilage, undergo mechanical solicitation under their normal performance in human body. In this sense, it seems necessary that proper tissue engineering strategies of these tissues should incorporate mechanical solicitations during cell culture, in order to properly evaluate the influence of the mechanical stimulus. This work reports on a user-friendly bioreactor suitable for applying controlled mechanical stimulation--amplitude and frequency--to three-dimensional scaffolds. Its design and main components are described, as well as its operation characteristics. The modular design allows easy cleaning and operating under laminar hood. Different protocols for the sterilization of the hermetic enclosure are tested and ensure lack of observable contaminations, complying with the requirements to be used for cell culture. The cell viability study was performed with KUM5 cells.
|
巻・号 |
15(2)
|
ページ |
471-8
|
公開日 |
2016-4-1
|
DOI |
10.1007/s10237-015-0698-5
|
PII |
10.1007/s10237-015-0698-5
|
PMID |
26153426
|
MeSH |
Animals
Bioreactors*
Cartilage, Articular / growth & development*
Cell Line
Electricity
Equipment Design
Mice
Reproducibility of Results
Software
Tissue Engineering / instrumentation*
Tissue Engineering / methods*
|
IF |
2.527
|
引用数 |
6
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
BIOPHYSICS
|
リソース情報 |
ヒト・動物細胞 |
KUM5(RCB2322) |