論文 - 詳細
| RRC ID | 39047 |
|---|---|
| 著者 | Fukao M, Kondo E, Nishino H, Hattori R, Horie A, Hashimoto Y. |
| タイトル | Presence of an H+/Quinidine Antiport System in Madin-Darby Canine Kidney Cells. |
| ジャーナル | Eur J Drug Metab Pharmacokinet |
| Abstract |
BACKGROUND AND OBJECTIVES:We have recently found an H+/quinidine antiport system in human kidney HEK 293 cells. The aim of the present study was to evaluate whether the H+/quinidine antiport system is expressed in Madin-Darby canine kidney (MDCK) cells. METHODS:We investigated the uptake and efflux of quinidine in MDCK cells. RESULTS:The uptake of 100 µM quinidine into MDCK cells was decreased by acidification of extracellular pH or alkalization of intracellular pH. In addition, the uptake of quinidine was highly temperature sensitive, but was extracellular Na+ and membrane potential independent. Furthermore, tetraethylammonium, a typical substrate of renal organic cation transporters, did not inhibit the uptake of quinidine in MDCK cells. On the other hand, lipophilic cationic drugs, such as clonidine, bisoprolol, diphenhydramine, pyrilamine, and imipramine, significantly decreased the uptake of quinidine in MDCK cells. The uptake of quinidine was saturable, and the Michaelis-Menten constant was estimated to be approximately 0.5 mM. In addition, the efflux of quinidine from MDCK cells was increased by the acidification of extracellular pH, suggesting that the transport system mediates not only the uptake, but also secretion of quinidine. CONCLUSIONS:The present findings suggested that the renal new antiport system is involved in the bidirectional membrane transport of quinidine in MDCK cells. |
| 巻・号 | 41(6) |
| ページ | 819-824 |
| 公開日 | 2016-12-1 |
| DOI | 10.1007/s13318-015-0314-1 |
| PII | 10.1007/s13318-015-0314-1 |
| PMID | 26628429 |
| MeSH | Absorption, Physiological / drug effects Animals Anti-Arrhythmia Agents / metabolism* Antiporters / antagonists & inhibitors Antiporters / metabolism* Biological Transport / drug effects Clonidine / pharmacology Dogs Epithelium / drug effects Epithelium / metabolism* Hot Temperature / adverse effects Hydrogen-Ion Concentration Kidney / drug effects Kidney / metabolism* Kinetics Madin Darby Canine Kidney Cells Membrane Transport Modulators / pharmacology Quinidine / metabolism* Tritium |
| IF | 1.913 |
| 引用数 | 2 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |