RRC ID 45727
著者 Fenker KE, Hansen AA, Chong CA, Jud MC, Duffy BA, Norton JP, Hansen JM, Stanfield GM.
タイトル SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans.
ジャーナル Dev Biol
Abstract Motility of sperm is crucial for their directed migration to the egg. The acquisition and modulation of motility are regulated to ensure that sperm move when and where needed, thereby promoting reproductive success. One specific example of this phenomenon occurs during differentiation of the ameboid sperm of Caenorhabditis elegans as they activate from a round spermatid to a mature, crawling spermatozoon. Sperm activation is regulated by redundant pathways to occur at a specific time and place for each sex. Here, we report the identification of the solute carrier 6 (SLC6) transporter protein SNF-10 as a key regulator of C. elegans sperm activation in response to male protease activation signals. We find that SNF-10 is present in sperm and is required for activation by the male but not by the hermaphrodite. Loss of both snf-10 and a hermaphrodite activation factor render sperm completely insensitive to activation. Using in vitro assays, we find that snf-10 mutant sperm show a specific deficit in response to protease treatment but not to other activators. Prior to activation, SNF-10 is present in the plasma membrane, where it represents a strong candidate to receive signals that lead to subcellular morphogenesis. After activation, it shows polarized localization to the cell body region that is dependent on membrane fusions mediated by the dysferlin FER-1. Our discovery of snf-10 offers insight into the mechanisms differentially employed by the two sexes to accomplish the common goal of producing functional sperm, as well as how the physiology of nematode sperm may be regulated to control motility as it is in mammals.
巻・号 393(1)
ページ 171-82
公開日 2014-9-1
DOI 10.1016/j.ydbio.2014.06.001
PII S0012-1606(14)00297-8
PMID 24929237
PMC PMC4134694
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism GABA Plasma Membrane Transport Proteins / biosynthesis GABA Plasma Membrane Transport Proteins / genetics GABA Plasma Membrane Transport Proteins / physiology* Hermaphroditic Organisms Male Membrane Proteins / metabolism Morphogenesis Mutation Sperm Motility / genetics Sperm Motility / physiology* Spermatogenesis Spermatozoa / cytology*
IF 2.896
引用数 8
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3813